Twilight's first time was a magical, enchanted event. In the literal sense, unfortunately.

Applejack tries to write a love letter and mostly fails. A TwiJack story.

After falling prey to a prankster, Luna gets revenge the only way she knows how. Murder.

After breaking her wing and being grounded for a couple days, Rainbow Dash gradually finds she's a lot smaller than she realized.

Twilight Sparkle decides to kill her friends. But, like, in a non-permanent 'you'll thank me for this later' kind of way.

Twilight Sparkle has turophobia... and her friends decide to help.

Scootaloo has to use the bathroom. The universe doesn't seem to want her to.

Queen Chrysalis, last of the changelings, monitors the hive and her children, as she has done many times before. However, something is bothering her.

Trixie recruits Lyra to help her with a spell. The results are...not what she had intended.

What happens when you got the wrong impression the first time around? Twilight learns from Gilda....