The ponies felt empty. Like their friendship was incomplete, since the element of laughter was gone broke the somber silence, "Now you all know the process of dying via suffocation even better than before. Although no one here knows it like Pinkie Pie did! Heeeeheeeeheeeee."

So much rage was directed towards the mare murderer. Rarity expressed her fury, "If I had my horn...you...you...*sob*" She bitterly wept.

AJ spoke, "If we ever get out of this..." She swallowed, "You are no longer our friend... *sniff*"

Dash was in a state of extreme trauma, "Dead...d-d-dead," She was shaking and crying.

Twilight found something that amused her, "Aw, Fluttershy is still out cold. I'll have to fill her in on what she missed. HA!"

Applejack noticed Twilight's sudden personality change. "HOW IN THE HAY CAN YOU LAUGH ABOUT THIS?"

"HAHAHAHAHAHAHA! Oh AJ." Twilight took off her surgical mask to reveal an impossibly wide, demented clown like smile. "Let me show you why I laugh."

Twilight pressed her face against Apple's chest, breathing heavily into it, "Such beautiful, powerful muscles. Oh. I must know more of them." Moving her self away from AJ, she brought many sharp ended, needle sized rods of various lengths, with small bulbs on their tops, to the farm horse. With her glowing horn, she inserted rods of specific lengths into specific areas in AJ's body, with specific angles, with great precision. So many inserted rods were covering the orange pony. She didn't bleed a lot though, because the tips of the robs were extremely sharp. The twisted scientist sat behind the panel of buttons. She magically uncuffed Applejack. AJ fell to the floor, then stood up in disbelief. Was she free?

Thoughts were racing through her head of what move she should make. She quickly tried to set her friends free. Everyone was grateful for another chance of getting out of this living hell. She tried opening the cuffs. It did not work. She attempted to pull them out of the cuffs. But they were too firmly held in place. She lunged at Twilight, "Set them free, or you will rue-" AJ suddenly lost control of her body. She felt like the bulbs on the needles in her were sending jolts of electricity through her muscles. Then she noticed twi's horn radiating light, causing a lot of buttons and dials to be pushed and turned on the central board.

Twilight gave a confirmation, "Yep. I now control almost every muscle in your body responsible for voluntary movement." Rainbow and Rarity feared for their friend. What did Twilight have in store for Applejack. "HAHAHAHA! Hoh ho haha hehu! Now do you see the funny side?" Twilight said as she forced a smile on Applejack's face. Twilight's mouth then retained its usual form. "Aw, I'm only fooling around with ya. Anyway, enough tomfoolery. Let us move on to lesson zero, ur, two."

Twilight had her puppet walk into a back room. AJ tried to fight it. But it was futile. She was pushing a heavy bulk of machinery back to the main room. It had a square metal platform with a pole shooting straight up from one of it's sides, supporting an identical square metal platform lined up precisely, and straight above the bottom metal plate. And there was a meter measuring weight in newtons, at the very top of the pole. Twilight took a small controller that was connected to the bottom of the pole via an electric cord.

Twi had Apple stand on her two hind legs on the square platform with the other platform above her, and elaborated, "Our next test subject is Applejack, who has apples for a cutie mark. That signifies that she, as a farmer, grows, bucks, sometimes cooks, and sells apples. She must be rather physically strong. But how strong? What is her limit? Well, using this device in my hooves, I will have the top platform push down on Applejack with a force equivalent to the weight of an object with a certain mass. The mass will be measured in kilograms. And the weight will be measured in newtons, which is mass in kilograms, times the acceleration of gravity, which is 9.8 meters per second per second. And the meter at the top of the pole will show the force of the simulated weight in newtons, pressing against AJ's body. It's AJ's job to hold up the platform. Our goal here is to find out just how much weight she can lift before she breaks, as I add to the force little by little. Newton by newton. Let's start with say... 300N. And here wwweeee begin!... Oh. One question, AJ. Do you still think of me as a friend? Answer honestly."

"I don't own you any answer!" replied the orange, pigtailed pony.

"Applejack. Answer me, and I'll set you free."

"...I... I don't know..."

"Thank you for you truthful answer, element of honesty. I, however, am not that element. I'm not going to set you free."

"I figured that."

Suddenly, AJ felt her mouth and tongue move against her will. "Of course I'm your pal Twi. I'm so grateful we're doing this together. But I do hate these three other losers."

Rainbow and Rarity did not expect this from Applejack. Twilight informed them, "Oh, don't worry. She still loves you all dearly. I made her say that with these rods. Just having some fun. Teehee."

She pressed a button and turned a dial on the control device in her hoof. The top plate was pushing down on the reluctant pony. All Rainbow Dash and Rarity could do was to watch in dread. Their hearts were racing. They did not want to lose another chance to break free from this nightmare. "Don't let that jerk keep control over you! Show her who's boss!" Rainbow Dash cheered AJ on.

"We know you can fight it!" Rarity said, trying to boost enough confidence into the struggling pony. AJ was trying her best to move away from under the square metal plate. But the bioelectricity from the rods were to strong. She had no choice but to have Twilight force her to push up on the platform.

300 newtons were no problem. "Very good. You're stronger than I thought." The experimenter mocked. She turned the dial and the meter at the top read 445N. "More." Twilight turned the knob and the meter went up to 574N. It was now getting a bit hard to hold it up.

"Add a little more and you count to four," Twilight happily sang. "Remember that? Oh, that Pinkie Pie!" Twilight turned the dial. 671N. AJ was getting sore. She turned the dial continuously without stopping, but at a very slow pace. The arrow on the meter moved passed the 712N mark. Than the 756N and 801N marks. As it hit the 885N mark Applejack's muscles were incredibly sore. Very, very tired and in such pain, she wanted to just lie down on the floor and rest so badly, but that wasn't going to happen.

After seeing the amount of force she had to go against, AJ's friends doubted that she could leave the platform, or even survive. Were they about to lose another loved one? They didn't believe it. In just one night, their lives were turned completely upside-down. Their Pinkie Pie, tortured and killed. And now Applejack was on the verge of dying. Everything they thought they knew about their friendship with Twilight was washed down the drain. Just a few hours ago, they considered the purple unicorn as one of their closest, dearest friends. And now they were sad with her, angry with her, and afraid of her.

934N. 979N. She didn't know how much more she could take. The force was pushing her to her very limit! 1023N. 1080N. She felt she could not handle anymore. 1103N. 1134N. 1183N. She heard and felt her bones and spine crack. The others could not stand to watch, yet they couldn't turn their heads. Their eyes were filled with terror as they watched with horrible anticipation of what was about to happen.

Twilight wrote down on a scroll the weight at which AJ's skeleton started to break. Then she kept going. 1201N. 1245N. 1289N. Applejack was hurting greatly as her bones further broke under such pressure. It was as if she was having an enormous backache of immense pain that was progressively worsening. Twi turned the knob a little further till it reached 1339N. And, SNAP! AJ's back broke in two, "AAAOOOUUG!" Her fellow captives cringed and screamed at the sight. Oh, how they screamed!

She fell to the floor, only to have the top plate smash into her face and break her nose. Her screams of pain were muffled by the crushing metal surface. Her skull bone as well as her rib cage cracked in several areas. The Unicorn wrote down the force at which her spine broke in two, "1339N. Aaannnnd done!...hmm... I wonder." She continued to increase the simulated weight. The pressure was so extreme, that the orange earth pony's ribs shattered into over a dozen pieces. The fragments punctured the lungs. She could no longer scream. She could only sound a muffled cry. "You should be very proud of yourself Apples." Applejack could barely hear the scientist speak. "You contributed your life to science. I know I'm proud of you."

There was massive internal bleeding. All she could think about was the pain... until her skull fractured into about 12 sharp pieces and cut through her brain. Her life functions faded. She shared the same fate as Pinkie Pie. Death.