Ms. Sparkle continued with the party pony. By now Pinkie had lost a significant amount of nutrients carrying bodily fluid, as was evident by the red puddle on the floor below her. Twilight used her hoof to lift one of the flaps of Pinkie's flesh and meat made by her huge Y shaped wound on her underside. She stretched, compressed, and wiggled the mass of meat and skin as if she were studying it. Pinkie let out a whine of great discomfort with each burning stretch and twist.

Fluttershy shouted, "LEAVE PINKIE ALONE! You're hurting her... badly! Just listen to her cries of pain. Doesn't that say anything to you?"

"Oh, she's just being Pinkie Pie," was Twilight's response.

Fluttershy continued, "Why did you cut Pinkie's belly open? She's your friend!"

"I told you. This is for science," Twi answered. "She is my friend. That is why we will learn together. I opened Pink because if I'm going to remove her brain to study it, I may as well remove the rest of her organs first." The five ponies were utterly shocked at what they just heard.

Twilight than wore a gleeful face, "Once we're done learning about Pinkie Pie, then we shall move on to AJ, Dash, Shy, and then Rarity. When we are finished, more will be known about the magic of friendship than ever before!"

All of them were breathing heavily and fast. Their heart's were pounding. Panic was in the air. "I'll be back in a sec." said Twilight. They all shouted and yelled pleas for mercy just as Twilight left the room.

Upon returning, she dragged a huge multi-layered cart with an array of glass containers filled with a clear liquid. The five ponies were petrified at the freaky and unnerving sight before them. Some jars had active pony organs already inside of them. The hearts were still beating. The lungs were still expanding and compressing. Stomachs were still contracting their muscles. As if the liquid in the containers was some sort of bioelectricity inducing substance. The glass bottles had labels on them. They labeled the type of organs that were in them, and ponies they belonged to. The horrified mares read the labels. Some empty ones (but still with liquid) read Pinkie Pie. Some containing organs read Blue October, Pokey Pierce, and... Apple bloom!

Applejack's world was further crashing down, "YOU KILLED HER! YOU KILLED MY KID SISTER! SHE'S WAS JUST A LITTLE HELPLESS FILLY!" Tears were flowing down her cheeks.

The killer explained, "Well, yeah. I needed practice before I got to Pinkie. Much of science is trial and error." AJ's throat moistened and she found it hard to swallow. All sorts of intense emotions ran through her. Shock. Fear. Despair. RAGE!

"You know," Twilight went on, "it's interesting, this taboo of society. Ponies are always more sensitive about young foals being hurt and killed. Why do you think that is?" Twilight looked at everyone, "And finally we can start the lesson." Twilight Grabbed a huge metal, silver nail and a hammer from the open metal case. Pinkie didn't want to know what the unicorn was going to use them for. "Twilight, no! NO! PLEASE NO!"

After resting the nail's tip on the inside of one of the flaps of meat, Twilight leaned over and kissed Pinkie on the forehead. Then she swung the hammer and it hit its target. The nail pinned down one of Pinkie Pie's flaps of skin to the table. The nail rested in a hole pre-embedded in the table.

"AAAAAAAAAAAAAAA," Pinkie felt a high concentration of activated pain receptors. Twi took two more out and hammered them to pin down that flap more securely. Pinkie let out a high pitched shriek with each sudden, blood squirting stab into the meat and flesh. Taking three more nails, she did the same to the flap on the pink pony's other side. This left Pinkie's ribcage and bottom organs exposed. Rarity felt nauseous. But Twilight wasn't finished.

She took out a small circular saw out of the box. It was a bone cutter. She turned it on and pushed the spinning saw into the bottom of Pinkie's blood covered sternum. The loud cutting noise could be heard throughout the dungeon. Sawing straight up through the hard chest bone, countless red drops sprayed onto Pinkie's tear soaked face and on Twilight's white surgeon's apron and mask.

The crying, begging, and pleading for the purple unicorn to stop, from the four other ponies, were relentless. Applejack pleaded, "ANYTHING! ANYTHING AT ALL! NAME IT. WE'LL DO IT. PLEASE JUST STOP THIS!"

Dash implored, "No, NO! NO MORE! NNNOOOO!"

Rarity shouted, "WE LOVE YOU PINKIE! WE'LL GET YOU OUT OF THIS! WE SWEAR!"

Fluttershy was of course crying the hardest, "Pinkie Pie is your friend! How could you do this to poor Pinkie? Please PUT HER BACK TOGETHER!" But they didn't seem to phase Twilight at all, except for her horn. Which looked as though it sharpened.

With Pinkie's sternum split, the surgeon unicorn inserted a rib spreader into the crevice and turned the lever enough to push the two half sternums apart, leaving Pinkie's beating heart and expanding and compressing lungs in plain sight. Pinkie was too frightened to look down. Twi locked the lever in place.

Next, she brought out what looked like a huge basting bulb and explained, "See this? It contains alcohol. I'm going to pour it all over your exposed internal organs to prevent infection. You don't expect me to experiment on infected organs, do you? This is going to sting... a lot. Brace yourself!"

"No... Twi... no...," Pinkie spoke weakly from blood loss, her face wet with blood and tears. As if Twilight didn't even hear her, she squirted the infernal liquid all over poor Pinkie Pie's bare insides. Sssstiiiinnnngggg! The pain was so intense, it was like nothing she had ever suffered before. Deafening, blood curdling screams of sheer agony echoed off the cave walls. "THIS IS NOT RRRIIIGGHHT!" cried the suffering mare.

Twilight repeatedly rubbed her cheek against her sobbing victim's, and gave some kisses in between, "Shh, shh, sh, shhhh. It's okay. It's going to be alright."

The four other captives were in such trauma. They couldn't stand seeing their best friend in so much pain. Pinkie looked so weak and helpless. It was too much for them to handle. Fluttershy threw up and blacked out.

Twilight removed her mask. "Now. This is when we learn more about Pinkie's mentality." She floated an linked feather and scroll to herself. Twi put her face right up to Pinkie's, "Hey, Pinkie Pie."

Pinkie weakly glanced at her captor. Miss Sparkle's eyes went wide as she spoke with a big, happy smile. "Guess what. I KILLED Applebloom! I killed her. And I'm going to KILL you too! And it makes me happy! Your cutie mark does not lie. Your parties do give ponies smiles. And what a party this is! Tell me, how does all this make you feel, emotionally? Does this change your view on this world we live in?" The unicorn waited for a response from the miserable pink pony.

After a few moments, the crying, pitiful pony started to sing, "G-g-g...g-giggle at the ghostie. G-guffaw at the grossly. Crack up at the c-cree-creepy. Whoo-whoop it up with the weepy. Chor-ch-chortle at the kooky. Snortle at the spooky-hy-hy-hy-hhhhyyyy."

When she finished, Twilight spoke with intrigue, "Interesting. You truly are the carrier of the element of laughter. But laughing can only help you so far in dire situations like this. And you need to learn that." Twilight's irises fluctuated between dark red and bright red, as she picked up the bone cutter. She started sawing around one of Pinkie's hind legs, just above the knee. Pinkie screamed from the painful slicing of her muscle. She felt like she could do nothing to stop this. Not even laughing.

With more blood splatter, the spinning blade cut the bone thin enough to snap, which is exactly what Twilight did. And the pink leg fell to the giant tree trunk floor, making another mess of blood. Twilight Sparkle squirted more stinging alcohol onto the newly made injury, and the severed leg. Putting the red dripping limb onto a tray on the cart, she asked, "Do you still think 'giggling at the ghosties' will save you now, Pink?"

Pinkie gave up hope. "...n... no..."

Twilight addressed everyone, making sure they were all listening. "That's it. We all learned something about our friend Pinkie Pie. And that is that, under such horrific circumstances like this, she concludes that laughter will not always make the monsters disappear. But we are not done. There is more to be learned about Pinkie."

When she was finished, she put back on her surgical mask, readied the glass containers, then sterilized her hooves. The ponies made one last attempt to reason with the heartless horse. Applejack tried first, "Twi. Ya don't have to do this. It's not too late. Just stitch Pink back together."

Dash spoke, "Yes, Twi. She's your friend. We know you really don't want to do this."

Rarity was next, "We don't know what has gotten into you, but please, let us help you. We can help. We love you dear."

Twilight was grateful for their offer, "Thank you all. You are my dear friends." There was a spark of hope in them. Twilight's eyes turned an even brighter red than before, "But there is nothing wrong with me." Everyone's heart sank. Then came the begging and sobbing again.

Twilight did not seem to care, as she opened the bottle and grabbed the party pony's stomach. Pinkie pie felt sick to the stomach, that is until Twilight gave it a good yank out of her, carrying with it part of the esophagus. "Uuugggk!" reacted Pinkie. Twilight immediately put the digestive organ into the preservative fluid filled jar and closed the lid.

Twilight made a discovery, "Well you had a big stomach. Which is to be expected, since you ate a lot of goods." Twilight went for her liver next. "This organ is rich with blood!"

Twilight proceeded with the pancreas, gallbladder, spleen, and small intestine, giving brief facts about them as she did. The mares could not believe nor understand what they were witnessing. Twilight Sparkle, their very close and dear friend, was, seemingly out of nowhere, removing the internal organs of an innocent fellow pony. Rarity's nausea was worsening till it was too much. Her stomach erupted and she vomited several times in a row, making a big, strong odored, mess of bile all over her body and the floor. At this point they felt that all hope was lost for their pink friend. They felt so sad. They loved Pinkie so much.

"Interestingly enough, Pinkie is so healthy. And considering how many sweets she consumes... yeah, I'll have to look into that. I'm guessing it's how she hops around a lot, using those muscles." Twilight said, briefly studying Pinkie's long intestine before folding it into a glass jar. Twilight dipped the bloody blade into a bowl of alcohol to clean it. After giving Pinkie's vulva a few good rubs, she used the scalpel to pierce the skin of the lying pony's groin. Pinkie could feel the very sharp tool easily dig deeper into her meat. The demented surgeon started carving out Pinkie's sensitive genitals, very slowly and carefully as not to damage any internal parts of the pink pony's sex organ. Pinkie's awareness faded.

Once finished, she lifted the blood dripping severed privet part with caution. The vulva, vagina, cervix, uterus, fallopian tubes, and ovaries, all still connected. "Reproduction. Very important." Twilight poured alcohol onto Pinkie's disembodied marehood and placed the entire reproduction system into a vacant container and sealed it tight. She then spilled the alcohol onto Pinkie's genitaless crotch, "Wake up Pink." The mutilated horse was very weak. But the sting still managed to wake her up. "You don't want to miss your last lesson in life!" said Twilight as she prepared for the final incision. "I'll remove your lungs and heart after, so you can witness the lecture on your brain before I take it out."

Sad, confused, crying, and in tremendous discomfort, Pinkie lost the will to hope. She was just waiting for it to end. A sharp painful pinching sensation was felt as the scalpel cut across the small pink horse's forehead and around the cranium. The wincing pony's forehead was oozing out red fluid. Twilight pealed off the flesh from the top of the head, taking a part of Pinkie Pie's poofy pink mane with it, causing her to scream. Next, using the bone cutter, Twi sawed through the skull bone along the incisions of the skin. Making a complete circle around the top of the head, she lifted the head bone piece off, exposing Pinkie's tender brain. Pinkie braced herself as Twilight squirted more alcohol onto the brain. The sensation was that of an immense, heavy, pounding headache all over the surface of the her thinking organ. And Twilight caressing it with her hoof wasn't helping.

Then the purple unicorn applied her magic to levitate a kind of brainwave analyzing device to her. She put it up to Pink's head, "Isn't this interesting. The left temporal lobe is just as active as the right. I expected the left side to be far less active. Would you ever forgive me for taking you to be a moron Pinkie? Hee, hee, hheeee.." Pinkie was too weak and in too much agony to be mad, or even care about the cold words escaping from her tormenter's mouth. "And thus concludes our learning of Pinkie."

The terrified mares knew what was coming. They could not bear to watch, yet they couldn't look away. Twilight Sparkle took hold of one of the lungs. Streams of tears profusely flowed down from Dash's eyes, "This... *sniff*... isn't the Twilight Sparkle we knew... *sob*" Twilight hesitated for a moment... but then pulled on the lung until it disconnected from Pinkie. Everyone cried even harder. Pinkie, already struggling to breathe, found it much harder to take in oxygen with only one lung. "Nos" and "pleases" reverberated throughout the basement laboratory. Twilight placed both of her hooves on the only lung Pinkie had left. Twilight was hesitant again. She started sweating and breathing heavily. Using her magic to levitate her fizzing liquid filled laboratory flask up to her mouth... she took a chug of it, and then forced out the last means Pinkie had to breathe.

Within about four minutes Pinkie passed out from lack of oxygen. They knew it. Her friends knew that in a few moments the inevitable would happen to the blood covered pony. After a short passing of time, they saw the beating of Pinkie Pie's visible heart stop. Her eyes slowly closed. The brain activity ceased. They so desperately didn't want to face the ugly fact. But they had no choice. Their friend Pinkie Pie... was dead.